PLASMID ISOLATION
Plasmid isolation is a fundamental molecular biology technique used to extract small, circular DNA molecules (plasmids) from bacterial cells. These plasmids often carry genes of interest, such as antibiotic resistance or recombinant DNA constructs. The process typically involves cell lysis, removal of proteins and genomic DNA, and purification of plasmid DNA using methods like alkaline lysis or spin column-based kits. The isolated plasmid DNA is then used for downstream applications such as cloning, sequencing, PCR, and genetic engineering.
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