Biotech/Pharma Summer Training Program 2024

Amplikon Biosystems, India organizes Biotech summer/Winter training/Internship (short term) program each year to enhance hands on practical knowledge of life sciences students with latest Industry oriented courses.

Amplikon Biosystems International Pvt. Ltd. is a leading ISO 9001:2015 certified company, with centers at Mumbai (Delhi - NCR), Mohali (PUNJAB),Ahmedabad, Dehradun,Hyderabad,Mumbai & Chennai.

• All the modules are industrially designed as per the requirements of Biotech sector.

• A Training Certificate (approved) will be issued with grades after successful completion of training program.

• The verification of the certificate will be provided for life. if you apply for further studies or job (India or Abroad) in an from the date of certificate issued.

Mammalian and stem cell culture ( Module 1 )

Introduction to Cell culture/Mammalian cell culture/ Stem cell culture. Transfection and single cell clonning in mammalian cell culture. Overview of cell culture for vaccine production, therapeutic proteins, gene therapy and cancer research, Biosafety recommendation on the handling of Mammalian cell cultures, Primary Cell culture and its maintenance, Contamination in cell culture, Basics of Adherent vs Suspension culture, Trypsinization, passaging for suspension culture (Shake off and scraping), Sterilization of all chemicals and instruments, Collection of blood samples and Cryopreservation, Isolation of Peripheral Blood Mononuclear Cells (PBMC) by sucrose gradient centrifuge, Preparation of PBMC suspension, Washing of PBMC isolates, Primary culture of PBMC into cell culture media, Handling and maintenance of CO2 incubator, Total viable cell counts through hemocytometer, Subculture of PBMC into culture media, Cell viability assay using MTT/alamar blue, Basic concept of Labelling, Culture and Handling of mammalian cell culture and its maintenance.Introduction to Cell culture/Mammalian cell culture/ Stem cell culture. Overview of cell culture for vaccine production, therapeutic proteins, gene therapy and cancer research, Biosafety recommendation on the handling of Mammalian cell cultures, Primary Cell culture and its maintenance, Contamination in cell culture, Basics of Adherent vs Suspension culture, Trypsinization, passaging for suspension culture (Shake off and scraping), Sterilization of all chemicals and instruments, Collection of blood samples and Cryopreservation, Isolation of Peripheral Blood Mononuclear Cells (PBMC) by sucrose gradient centrifuge, Preparation of PBMC suspension, Washing of PBMC isolates, Primary culture of PBMC into cell culture media, Handling and maintenance of CO2 incubator, Total viable cell counts through hemocytometer, Subculture of PBMC into culture media, Cell viability assay using MTT/alamar blue, Basic concept of Labelling, Culture and Handling of mammalian cell culture and its maintenance.

Assay Development in Biotechnology ( Module 2 )

Introduction and Scope

Introduction to assay development, emphasizing its importance and applications in biotechnology, covering types such as biochemical, cell-based, and molecular assays

Laboratory Safety and Good Laboratory Practices (GLP)

General and safety rules for working in the lab, including handling and disposal of hazardous materials, proper lab attire, and personal protective equipment (PPE).

Basic Laboratory Techniques and Instrumentation

Introduction to laboratory instruments such as spectrophotometers, plate readers, and PCR machines, including calibration and maintenance. Techniques for micropipette handling, including forward and reverse pipetting.

Biochemical Assays

Principles and applications of spectrophotometric assays, enzyme activity assays (e.g., amylase, protease), and protein quantification assays (e.g., Bradford, BCA). Preparation of reagents and buffers.

Cell-based Assays

Techniques for cell culture maintenance, cell viability assays (MTT, Trypan blue exclusion), reporter gene assays (e.g., luciferase, GFP), and high-throughput screening methods.

Molecular Assays

Methods for nucleic acid extraction and quantification (DNA/RNA), Polymerase Chain Reaction (PCR) and its variations (qPCR, RT-PCR), gel electrophoresis for nucleic acid analysis, and DNA/RNA hybridization techniques (Southern, Northern blotting).

Assay Optimization and Validation

Optimization of assay conditions (pH, temperature, ionic strength) for sensitivity, specificity, and reproducibility. Statistical methods for data analysis and interpretation, including validation and standardization of assay protocols.

Data Analysis and Interpretation

Use of software tools for data analysis (e.g., GraphPad Prism, Excel), preparation of standard curves, calculation of unknowns, and reporting and presentation of assay results.

Practical Applications and Case Studies

Review of successful assay development in the biotech industry, troubleshooting common assay problems, applications in drug discovery and development, and future trends in assay development.

Hands-on Laboratory Exercises

Practical sessions on enzyme activity assays, cell viability and proliferation assays, PCR and gel electrophoresis experiments, and data analysis workshops

Cell Culture Basics : Principles of mammalian cell culture, media preparation, and aseptic techniques. Monoclonaal Antibody production , Clone Confirmation by Single cell Clonning , lipofectamine Based Transfection ,

Bioreactor Operation : Types of bioreactors, setup, and operation procedures.

Monitoring and Control : Methods for monitoring cell growth, viability, and metabolism.

Scale-Up Processes : Techniques for scaling up from lab to pilot-scale bioreactors.

Troubleshooting : Common issues in cell culture and bioreactor operations and their solutions.

Industrial Downstream processing

Course Topics:

Introduction to Protein Purification

Overview of protein purification strategies.

Importance and applications of recombinant protein purification.

Basics of Chromatography

Principles of chromatography.

Types of chromatography used in protein purification

Column packing and preparation.

Ion Exchange Chromatography (IEX) – Theory

Principles of ion exchange chromatography.

Types of ion exchange resins (anion and cation exchange).

Factors affecting protein binding and elution.

Ion Exchange Chromatography (IEX) – Practical

Buffer preparation and column equilibration.

Sample loading and washing steps.

Gradient elution and fraction collection.

SDS-PAGE analysis of IEX fractions.

Optimization of Ion Exchange Chromatography

pH and ionic strength optimization.

Troubleshooting common issues.n

Case studies and examples.

Affinity Chromatography – Theory

Principles of affinity chromatography.

Types of affinity tags (His-tag, GST-tag, etc.).

Ligands and matrix selection.

Affinity Chromatography – Practical

Buffer preparation and column equilibration.

Sample loading and washing steps.

Elution strategies and fraction collection.

SDS-PAGE analysis of affinity purification fractions.

Optimization of Affinity Chromatography

Imidazole concentration optimization.

Reducing non-specific binding.

Troubleshooting common issues.

Advanced Techniques and Applications

Combining IEX and affinity chromatography for enhanced purity.

High-throughput purification techniques.

Purification of multi-protein complexes.

Data Analysis and Interpretation

Analyzing SDS-PAGE and Western blot results.

Activity assays for purified proteins.

Quality control and validation of purified proteins.

Case Studies and Real-world Applications

Case studies of protein purification projects.

Industrial and therapeutic applications

Cell and Molecular Biology ( Module 3 )

Vector Designing for Recombinat protein expression systems , mammalian gene expression vectors , CRISPER CAS9 Construct Designing , Lentivirus and Adenovirus vector designing , Codon Optimisation , Restriction Mapping , Introduction and scope of Cell and Molecular Biology, General and safety rules for working in the Lab, Demonstration of module-oriented instruments, Basic concept of percentage, molarity and normality, Principle-SOP and application of spectrophotometer, Composition of buffers (TAE and TBE). Overview of differential leukocyte cells, Preparation of blood smear and staining of blood cells, Genomic DNA/RNA isolation from plants, Genomic DNA/RNA isolation from bacteria. Basic concept for preparation of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution, Introduction and application of electrophoresis, Genomic DNA isolation from Hair/Nail/Blood, Qualitative analysis of DNA/RNA through Agarose Gel Electrophoresis. Genomic DNA quantification & purity check through UV-VIS spectrophotometer, Basic concept of PCR, Development of PCR assay, Performance of PCR in thermal cycler, Preparation of product labels, Types of PCR (RT-PCR, Gradient PCR, Nested PCR, and its application) (T).

Pharmaceutical Quality Control & Quality Assurance ( Module 4 )

Introduction and job prospects of pharmaceutical quality control. GLP in Pharmaceuticals, General and safety rules for working in the Lab, Demonstration of instruments, Clean room concept, Micropipette handling (forward and reverse pipetting techniques), Estimation of different tablet hardness through tablet hardness tester, Basic about the limit test in pharma, Limit test of heavy metal (lead), limit test of sulphate, limit test of chloride, Qualitative analysis of iodoform/oxalic acid/ester, Introduction about Minimum Inhibitory Concentration (MIC), MIC of antibiotics against pathogenic bacteria, Qualitative Assay of Acetaminophen/Acetylsalicylic Acid tablets, Principle-SOP and application of spectrophotometer, Percentage purity of Paracetamol from different brands of through UV-VIS spectrophotometer, Overview of the flame photometer, Analysis of different salts using a flame photometer, Principle-SOP and application of Karl Fischer Titration, Basic concept of Moisture analysis from different tablets through Karl Fischer Titration.

Nano Biotechnology ( Module 5 )

Introduction about Nano-Biotechnology and its application. General and safety rules for working in Lab, Demonstration of instruments, Good laboratory practices (GLP), Basic concept of calculation (Percentage, Molarity and Normality), Micropipette handling (forward and reverse pipetting techniques), SOP of magnetic stirrer with hot plate, Different methods for the preparation of nanoparticles, Synthesis of Ag-nanoparticles/Cu-nanoparticles/Mg- nanoparticles through chemical reduction methods, Screening of medicinal plants for green synthesis of nanoparticles, Green synthesis of Ag- nanoparticles/Cu- nanoparticles/ Mg- nanoparticles through different medicinal plants, Principle-SOP and application of spectrophotometer, Characterization of different nanoparticles through UV-VIS spectroscopy scanning method, Different method for the detection of antimicrobial activity, Biological screening of different Nanoparticles (Antimicrobial Susceptibility Testing (AST)/Antioxidant activity).

Instrumentation Techniques ( Module 6 )

General and safety rules for working in Lab, Demonstration of all instruments, Good laboratory practices, Aim, principle, SOP and Calibration of micropipette, reverse and forward pipetting; Aim, principle, SOP, Calibration and application of autoclave; Aim, principle, SOP, Calibration and application of pH meter; Aim, principle and Calibration of lactometer; Aim, principle, SOP and application of Soxhlet apparatus, Aim, principle, SOP and application of Clevenger apparatus; Aim, principle, SOP and application of TDS meter, Aim, principle, SOP and application of viscometer; Aim, principle, SOP and application of vortex mixer; Aim, principle, SOP, Calibration and application of LAF; Aim, principle, SOP, Calibration and application of centrifuge; Aim, principle, SOP, Calibration and application of UV-VIS Spectrophotometer; Aim, principle, SOP, Calibration and application of UV-VIS Spectrophotometer; Aim, principle, SOP, Calibration and application of Agarose gel electrophoresis; Aim, principle, SOP, Calibration and application of SDS-PAGE; Aim, principle, SOP and application of colony counter, Aim, principle, SOP and application of microscope; Aim, principle, SOP and application of PCR, Aim, principle, SOP, Calibration and application of Flame photometer; Aim, principle, SOP and application of TLC, Column chromatography and HPLC; Aim, principle, SOP and application of Fermenter; Aim, principle, SOP and application of ELISA; Aim, principle, SOP and application of RIA (T) etc.

Medical and Clinical Microbiology ( Module 7 )

Introduction of medical and clinical microbiology, Overview and types of Sterilization techniques, Basic principle-Standard Operating Procedure (SOP)-application of instruments (Autoclave, pH meter, Laminar air flow, incubator, microscope and colony counter), Classification of bacteria based on Gram staining, Concept of basic calculation and safety rules for working in the Lab, Knowledge about BSL Labs and Classification, Types of media and solution preparation, Preparation and sterilization of selective media (EMB/ MSA/ MacConkey agar etc.), Isolation of pathogenic bacteria (Salmonella sp. Staphylococcus sp. Enterococcus sp. Proteus sp., E. coli, etc), Inoculation of pathogenic bacteria into nutrient broth medium, Different methods for the detection of antimicrobial activity, Basic concept of positive and negative controls, Antimicrobial activity test against isolated pathogenic bacteria by disc diffusion method, Minimum inhibitory concentration (MIC) via Agar diffusion method, Minimum bactericidal concentration (MBC) through Broth dilution method. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Genomics & Recombinant DNA Technology ( Module 8 )

Introduction of Recombinant DNA Technology (RDT), Application of RDT, Recent trends and future perspectives in RDT, Good laboratory practices (GLP), Basic instrument handling, Concept of basic calculation (Percentage, Molarity and Normality), Micropipette handling (forward and reverse pipetting techniques), Competent cell preparation, Introduction about ligation, T4 DNA Ligation of λ DNA by HindIII restriction enzyme, Overview of Agarose gel electrophoresis, Preparation of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution. Observe the efficiency of the ligation reaction through Agarose gel electrophoresis, Overview of Transformation, Transformation of ligated DNA, Learn restriction-digestion technique (Plasmid DNA cut with EcoR1 and HindIII), Agarose Gel Electrophoresis of digested plasmid DNA, Basic concept of DNA fingerprinting by restriction fragment length polymorphism (RFLP) and Blue-white screening. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Proteomics ( Module 9 )

Introduction and application of proteomics, Good Laboratory Practices (GLP), Basic instrument handling, Concept of basic calculation (percentage, molarity, and normality), Reagent preparation and Micropipette handling (forward and reverse pipetting techniques), Different basic tests for protein analysis, Screening of protein-rich sources for protein estimation, Principle-Standard operating procedure (SOP) and application of spectrophotometer, Estimation of protein through UV-visible spectroscopy, Standard curve preparation and Data Interpretation. Extraction of protein from different sources, Different methods of protein precipitation, Basic concept of reagents preparation for SDS-PAGE- Stacking gel - running gel - sample loading dye- tank buffer- Staining solution - destaining solution, Protein separation through vertical gel electrophoresis (SDS-PAGE) technique, Concept of staining and destaining technique in SDS-PAGE, Staining and distaining of SDS-PAGE gel and observation, Overview of protein analysis by Western blotting technique and the difference between SDS-PAGE, Native PAGE and 2D PAGE (T). (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Hyderabad, Jaipur, Dehradun and Gwalior)

Diabetes Single Nucleotide Polymorphism (SNP) Mutation Analysis ( Module 10 )

Introduction and scope of Molecular Biology, Overview of genomic DNA extraction, Bio-safety rules for working in a clinical lab, Demonstration of module-oriented instruments, Basic concept of molecular biology mathematics, Overview of Applied Molecular Biology, Basic handling and disposal of potential infectious/ clinical waste, Genomic DNA extraction from Blood, DNA sample quantification and qualitative analysis, Difference between Single Nucleotide Polymorphism and Mutations (Basic concept), Genetic risk factors for Diabetes and Molecular Diagnostics, SNP Polymerase Chain Reaction (SNP-PCR), SNP-PCR Amplicon gel electrophoresis, Data compilation, recording and interpretation, SNP analysis: Programs for prediction of Single Nucleotide Polymorphism effects. (Available at- NOIDA, Ahmedabad, Mumbai, Hyderabad, Jaipur and Dehradun)

Enzyme and Microbial Technology ( Module 11 )

Introduction and application of enzymology, Enzyme market size & major companies in India, Good Laboratory Practices (GLP), Basic instrument handling, Micropipette handling (forward or reverse pipetting techniques). Concept of basic calculation of percentage, molarity, and normality, Classification of media and solution preparation, Principle-standard operation procedure and application of instruments (pH meter, autoclave, colony counter, and laminar airflow), Screening of microbes producing extracellular hydrolytic enzymes (Amylase/Protease/Lipase/Tannase etc.) from different source (soil/water/industrial effluent etc). Subculturing of enzyme producing bacteria on culture media, Preparation of seed culture for fermentation media, Overview of fermentation and amylase enzyme, Preparation of fermentation media for amylase enzyme, Production of enzyme using submerged fermentation method (for amylase enzyme). Extraction of crude amylase enzyme, Determination of amylase enzyme activity using biochemical test, Enzyme/cell immobilization technique through sodium alginate and calcium chloride method. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Food Microbiology ( Module 12 )

Introduction to food microbiology and Microbial Limit Test (MLT), Good laboratory practices (GLP), Basic instrument handling and micropipette handling (forward and reverse pipetting techniques), Application and calibration/validation (Autoclave, pH Meter, Laminar Air Flow, Colony Counter, Incubator, and Microscope), Review of microbiological logbook entries, Concept of basic calculation (percentage, molarity, and normality), Types of media and solution preparation, Overview and types of sterilization techniques, Classification of bacteria based on Gram staining, Preparation of solid media for Diarrhea-causing bacteria, Screening of Diarrhea-causing bacteria on selective media from spoiled food samples, Introduction about streaking and subculturing, Streaking technique used for isolation of bacteria. Characterization of Diarrhea causing bacteria by staining technique, Basics of biochemical test, Identification of isolates using different biochemical test (Indole test/ Citrate test/ Urease test etc.), Antimicrobial susceptibility test against Diarrhea causing bacteria, Methylene Blue Reductase Test (MBRT) in milk. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Biochemistry & Immunology ( Module 13 )

Introduction to Biochemistry, Difference between antigen-antibody, Types of antibodies and basic instrument handling, Calculation (Percentage, Molarity and Normality), Micropipette handling (Reverse and Forward pipetting), Standard operating procedure (SOP) and application of different instruments (pH meter and spectrophotometer), Measurement of рН of biological liquids with universal indicator paper, Qualitative determination of Vitamin (A/C/D), Biuretic reaction for detection of protein, Qualitative test for the presence of carbohydrates (Molisch/ Tollen’s/ Fehling test), Quantitative determination of vitamin Р in the different samples via UV-VIS Spectrophotometer, Determination of alpha-Amylase activity (Serum/Saliva) on Starch, Components of blood. Bleeding time calculation, Clotting time estimation. Separation of Serum from human blood, Basic concept of antigen and antibody, Overview of enzyme-linked immunosorbent assay (ELISA), Direct Immuno enzymatic determination of thyrotropin/Hepatitis B in human serum through (ELISA) reader, Scientific approaches of ELISA and RIA technique. (Available at- NOIDA, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur and Dehradun)

Applied Industrial Microbiology ( Module 14 )

Industrial microbiology application & major products, Major companies in India, Scope & job prospects, Good laboratory practices (GLP), Demonstration of module-oriented instrument, Different types of sterilization techniques in microbiology, Basic principle-Standard Operating Procedure (SOP) and Application of the instruments (Autoclave, pH meter, Laminar air flow, incubator, microscope and colony counter), Classification of bacteria based on Gram staining, Types of media and solution preparation, Basic calculation (Percentage, Molarity and Normality), Overview of serial dilution, Serial dilution of soil sample, Basic calculation of Colony.

Bioinformatics (Module 15)

Introduction of bioinformatics, Application of bioinformatics, Scope of bioinformatics, Uses &Importance of bioinformatics, Sequence database similarity searching tools: FASTA, BLAST (BLASTP, BLASTN, BLASTX, TBLASTX, TBLASTN), Multiple sequence alignment tool: ClustalW, Introduction of Biological databases: Primary databases & Secondary databases, Sequence databases (GenBank, EMBL, DDBJ), Protein structure, classification and family databases (PDB, CATH, SCOP, Pfam, PIR,PROSITRE, Swiss-Prot), Detailed Understanding of Phylogenetic Analysis. . (N,Ah,M,Mu,H, L,C,D)

Microbiology (Module 16)

Basic of Microbiology, Good laboratory practices (GLP), concept of basic calculation, solution preparation, media preparation, Overview and types of Sterilization techniques, Basic principles, standard operating procedure (SOP) and application of instruments (Autoclave, pH meter, Laminar air flow, incubator and microscope) Classification of bacteria on the basis of Gram staining, types of media, Difference between gram positive and gram negative bacteria, Solid and liquid media preparation, Serial Dilution techniques, Isolation of bacteria from soil sample, Isolation of pure bacterial colonies, Gram staining technique for bacteria, Motility check with hanging drop method, Lactophenol Cotton Blue (LPCB) staining for fungus, Methylene Blue Reductase Test in Milk. (N,M, L,Mu,Ah,,B,H,C,D)

Molecular Techniques (Module 17)

Basic of Molecular Biology, Good laboratory practices (GLP), Overview of genomic DNA, Basic instrument handling, Basic concept of Percentage, Basic concept of Molarity and Normality, Micropipette handling (forward and reverse pipetting techniques), Principal, SOP and application of spectrophotometer, Composition of buffers, Extraction of genomic DNA from onion/banana, Extraction of genomic DNA from Bacteria, Extraction of genomic DNA from Plant, types of electrophoresis, Qualitative Analysis of DNA-Agarose Gel Electrophoresis, Quantitative Estimation of the DNA through UV-VIS spectrophotometer, RNA extraction (T) (N,M, L,Mu,Ah,,B,H,C,D)

Natural Product Research (Module 18)

Basics concepts of Natural Product Research , Safety of herbal medicine, Efficacy of herbal medicine, General and safety rules for working in Lab, Basic instrument handling, Concept of basic calculation, solution preparation, types of extraction, Preparation of standardized extracts through maceration, Introduction about phytochemicals, Qualitative Phytochemical analysis (saponin, tannin, flavonoids and alkaloids) of herbal extracts, types of media, Media preparation and Antimicrobial susceptibility tests (AST) of plant extracts against bacteria, Basic concept of Minimum Inhibitory Concentration test, Basics of chromatography, Thin layer chromatography (TLC) of crude extracts, Antioxidant Potential analysis of Bioactive extracts. (N,M, L,Mu,Ah,,B,H,C,D)

Food Technology (Module 19)

Basic of food technology, Good laboratory practices (GLP), Demonstration of instruments, concept of basic calculation, Micropipette handling (forward and reverse pipetting techniques), To extracts caffeine using polar-nonpolar solvent extraction technique and its confirmatory test, quality analysis of different Fruit Juices (pH, total moisture, solid content), overview of adulteration and this disadvantages according to FSSAI guidelines, Adulterant analysis in different brands of milk (Starch test, water test, formalin test, soap test and sulphate), Spectrophotometric Quantification of carbohydrates in different food samples, Quality analysis of turmeric powder (metanil yellow, yellow lead salt and starch test), Basic concept of chromatography, Thin Layer Chromatography (TLC). (N,M, L,Mu,Ah,,B,H,C,D)

Biochemistry (Module 20)

Basic of Biochemistry, Good laboratory practices (GLP), Basic instrument handling, Concept of basic calculation, Micropipette handling (forward and reverse pipetting techniques), To perform the isoelectric precipitation of casein present in milk, Introduction and types of titration, To Estimate the Saponification value in fats/oils, Classification of carbohydrates, Qualitative analysis of carbohydrates, Analysis of lactose by mucic test and microscopic view of mucic acid crystals, Biuretic reaction for detection of peptide bond, Principal of DNS reagent, Principle, SOP and application of spectrophotometer, Quantitative estimation of Carbohydrate by DNS Method, standard graph preparation and data interpretation. (N,M, L,Mu,Ah,,B,H,C,D)