Biotech/Pharma/ Training Program 2025

Amplikon Biosystems, India organizes Biotech summer/Winter training/Internship)  (short termprogram each year to enhance hands on practical knowledge of life sciences students with latest Industry oriented courses.

Amplikon Biosystems International Pvt. Ltd. is a leading ISO 9001:2015 certified company, with centers at Mumbai (Delhi - NCR), Mohali (PUNJAB),Ahmedabad, Dehradun,Hyderabad,Mumbai & Chennai.

1. All the modules are industrially designed as per the requirements of Biotech sector.

2. A Training Certificate (approved) will be issued with grades after successful completion of training program.

3. The verification of the certificate will be provided for life. if you apply for further studies or job (India or Abroad) in an from the date of certificate issued.

Mammalian and stem cell culture ( Module 3 )

Introduction to Cell culture/Mammalian cell culture/ Stem cell culture. Transfection and single cell clonning in mammalian cell culture. Overview of cell culture for vaccine production, therapeutic proteins, gene therapy and cancer research, Biosafety recommendation on the handling of Mammalian cell cultures, Primary Cell culture and its maintenance, Contamination in cell culture, Basics of Adherent vs Suspension culture, Trypsinization, passaging for suspension culture (Shake off and scraping), Sterilization of all chemicals and instruments, Collection of blood samples and Cryopreservation, Isolation of Peripheral Blood Mononuclear Cells (PBMC) by sucrose gradient centrifuge, Preparation of PBMC suspension, Washing of PBMC isolates, Primary culture of PBMC into cell culture media, Handling and maintenance of CO2 incubator, Total viable cell counts through hemocytometer, Subculture of PBMC into culture media, Cell viability assay using MTT/alamar blue, Basic concept of Labelling, Culture and Handling of mammalian cell culture and its maintenance.Introduction to Cell culture/Mammalian cell culture/ Stem cell culture. Overview of cell culture for vaccine production, therapeutic proteins, gene therapy and cancer research, Biosafety recommendation on the handling of Mammalian cell cultures, Primary Cell culture and its maintenance, Contamination in cell culture, Basics of Adherent vs Suspension culture, Trypsinization, passaging for suspension culture (Shake off and scraping), Sterilization of all chemicals and instruments, Collection of blood samples and Cryopreservation, Isolation of Peripheral Blood Mononuclear Cells (PBMC) by sucrose gradient centrifuge, Preparation of PBMC suspension, Washing of PBMC isolates, Primary culture of PBMC into cell culture media, Handling and maintenance of CO2 incubator, Total viable cell counts through hemocytometer, Subculture of PBMC into culture media, Cell viability assay using MTT/alamar blue, Basic concept of Labelling, Culture and Handling of mammalian cell culture and its maintenance.

Assay Development in Biotechnology ( Module 4 )

Introduction to assay development, emphasizing its importance and applications in biotechnology, covering types such as biochemical, cell-based, and molecular assays. Introduction to laboratory instruments such as spectrophotometers, plate readers, and PCR machines, including calibration and maintenance. Techniques for micropipette handling, including forward and reverse pipetting. enzyme activity assays (e.g., amylase, protease), and protein quantification assays (e.g., Bradford, BCA). Preparation of reagents and buffers. Techniques for cell culture maintenance, cell viability assays (MTT, Trypan blue exclusion), reporter gene assays (e.g., luciferase, GFP), and high-throughput screening methods.Methods for nucleic acid extraction and quantification (DNA/RNA), Polymerase Chain Reaction (PCR) and its variations (qPCR, RT-PCR), gel electrophoresis for nucleic acid analysis, and DNA/RNA hybridization techniques (Southern, Northern blotting). Optimization of assay conditions (pH, temperature, ionic strength) for sensitivity, specificity, and reproducibility. Statistical methods for data analysis and interpretation, including validation and standardization of assay protocols. Use of software tools for data analysis (e.g., GraphPad Prism, Excel), preparation of standard curves, calculation of unknowns, and reporting and presentation of assay results. Review of successful assay development in the biotech industry, troubleshooting common assay problems, applications in drug discovery and development, and future trends in assay development.Practical sessions on enzyme activity assays, cell viability and proliferation assays, PCR and gel electrophoresis experiments, and data analysis workshops

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Industrial Downstream processing ( Module 5) 

Course Topics Hands on Practical   

Introduction to Protein Purification, Importance and applications of recombinant protein purification.Basics of Chromatography, Principles of chromatography.Types of chromatography used in protein purification Column packing and preparation. Ion Exchange Chromatography (IEX) – Theory Principles of ion exchange chromatography. Types of ion exchange resins (anion and cation exchange). Factors affecting protein binding and elution. Ion Exchange Chromatography (IEX) – Practical ,Buffer preparation and column equilibration. Sample loading and washing steps. Gradient elution and fraction collection. SDS-PAGE analysis of IEX fractions. Optimization of Ion Exchange Chromatography pH and ionic strength optimization. Troubleshooting common issues.n Case studies and examples. Affinity Chromatography – Theory Principles of affinity chromatography. Types of affinity tags (His-tag, GST-tag, etc.). Ligands and matrix selection. Chromatography – Practical Buffer preparation and column equilibration. Sample loading and washing steps. Elution strategies and fraction collection. SDS-PAGE analysis of affinity purification fractions. Optimization of Affinity Chromatography , Imidazole concentration optimization. Reducing non-specific binding. common issues. Combining IEX and affinity chromatography for enhanced purity. High-throughput purification techniques. Purification of multi-protein complexes. Data Analysis and Interpretation Analyzing SDS-PAGE and Western blot results. Activity assays for purified proteins. Quality control and validation of purified proteins. Case Studies and Real-world Applications Case studies of protein purification projects. Industrial and therapeutic applications

Cell and Molecular Biology ( Module 6 )

  Vector Designing for Recombinat protein expression systems , mammalian gene expression vectors , CRISPER CAS9 Construct Designing , Lentivirus and Adenovirus vector designing , Codon Optimisation , Restriction Mapping , Introduction and scope of Cell and Molecular Biology, General and safety rules for working in the Lab, Demonstration of module-oriented instruments, Basic concept of percentage, molarity and normality, Principle-SOP and application of spectrophotometer, Composition of buffers (TAE and TBE). Overview of differential leukocyte cells, Preparation of blood smear and staining of blood cells, Genomic DNA/RNA isolation from plants, Genomic DNA/RNA isolation from bacteria. Basic concept for preparation of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution, Introduction and application of electrophoresis, Genomic DNA isolation from Hair/Nail/Blood, Qualitative analysis of DNA/RNA through Agarose Gel Electrophoresis. Genomic DNA quantification & purity check through UV-VIS spectrophotometer, Basic concept of PCR, Development of PCR assay, Performance of PCR in thermal cycler, Preparation of product labels, Types of PCR (RT-PCR, Gradient PCR, Nested PCR, and its application) (T).

Pharmaceutical Quality Control & Quality Assurance ( Module 7 )

Introduction and job prospects of pharmaceutical quality control. GLP in Pharmaceuticals, General and safety rules for working in the Lab, Demonstration of instruments, Clean room concept, Micropipette handling (forward and reverse pipetting techniques), Estimation of different tablet hardness through tablet hardness tester, Basic about the limit test in pharma, Limit test of heavy metal (lead), limit test of sulphate, limit test of chloride, Qualitative analysis of iodoform/oxalic acid/ester, Introduction about Minimum Inhibitory Concentration (MIC), MIC of antibiotics against pathogenic bacteria, Qualitative Assay of Acetaminophen/Acetylsalicylic Acid tablets, Principle-SOP and application of spectrophotometer, Percentage purity of Paracetamol from different brands of through UV-VIS spectrophotometer, Overview of the flame photometer, Analysis of different salts using a flame photometer, Principle-SOP and application of Karl Fischer Titration, Basic concept of Moisture analysis from different tablets through Karl Fischer Titration.

Nano Biotechnology ( Module 8 )

Introduction about Nano-Biotechnology and its application. General and safety rules for working in Lab, Demonstration of instruments, SOP of magnetic stirrer with hot plate, Different methods for the preparation of nanoparticles, Magnetic Nanoparticle Synthesis by Co-Precipitation , Iron Oxide Nanoparticle Synthesis , Silica and other Carboxy Coating on magnetic Nanoparticle , Using Coated Nanoparticle for Plasmid Isolation , Synthesis of Ag nanoparticles/Cu nanoparticles/Mg- nanoparticles through chemical reduction methods,Screening of medicinal plants for green synthesis of nanoparticles, Green synthesis of Ag- n anoparticles/Cu- nanoparticles/ Mg- nanoparticles through different medicinal plants, Principle-SOP and application of spectrophotometer, Characterization of different nanoparticles through UV-VIS spectroscopy scanning method, Different method for the detection of antimicrobial activity, Biological screening of different Nanoparticles (Antimicrobial Susceptibility Testing (AST)/Antioxidant activity).

Instrumentation Techniques ( Module 9 )

General and safety rules for working in Lab, Demonstration of all instruments, Good laboratory practices, Aim, principle, SOP and Calibration of micropipette, reverse and forward pipetting; Aim, principle, SOP, Calibration and application of autoclave; Aim, principle, SOP, Calibration and application of pH meter; Aim, principle and Calibration of lactometer; Aim, principle, SOP and application of Soxhlet apparatus, Aim, principle, SOP and application of Clevenger apparatus; Aim, principle, SOP and application of TDS meter, Aim, principle, SOP and application of viscometer; Aim, principle, SOP and application of vortex mixer; Aim, principle, SOP, Calibration and application of LAF; Aim, principle, SOP, Calibration and application of centrifuge; Aim, principle, SOP, Calibration and application of UV-VIS Spectrophotometer; Aim, principle, SOP, Calibration and application of UV-VIS Spectrophotometer; Aim, principle, SOP, Calibration and application of Agarose gel electrophoresis; Aim, principle, SOP, Calibration and application of SDS-PAGE; Aim, principle, SOP and application of colony counter, Aim, principle, SOP and application of microscope; Aim, principle, SOP and application of PCR, Aim, principle, SOP, Calibration and application of Flame photometer; Aim, principle, SOP and application of TLC, Column chromatography and HPLC; Aim, principle, SOP and application of Fermenter; Aim, principle, SOP and application of ELISA; Aim, principle, SOP and application of RIA (T) etc.

Medical and Clinical Microbiology ( Module 10 )

Introduction to medical and clinical microbiology, including a comprehensive overview of various sterilization techniques and their classifications, along with essential principles and Standard Operating Procedures (SOPs) for the effective use of laboratory instruments such as autoclaves, pH meters, laminar flow hoods, incubators, microscopes, and colony counters. This also covers the classification of bacteria based on Gram staining, basic calculations, and critical safety protocols for laboratory work. Additionally, there will be insights into BSL lab classifications, types of media, and their preparation methods, including the preparation and sterilization of selective media like EMB, MSA,and MacConkey agar. The curriculum will address the isolation of pathogenic bacteria, including Salmonella, Staphylococcus, Enterococcus, Proteus, E. coli, and others. Furthermore, it will explore the inoculation of pathogenic bacteria into nutrient broth mediums, various methods for detecting antimicrobial activity, and the fundamental concepts of positive and negative controls. Finally, students will engage in antimicrobial activity testing against isolated pathogenic bacteria using the disc diffusion method, alongside determining the Minimum Inhibitory Concentration (MIC) through the agar diffusion method and the Minimum Bactericidal Concentration (MBC) via broth dilution techniques.

Genomics & Recombinant DNA Technology ( Module 11 )

The course then explores various applications of RDT across fields like medicine, agriculture, industrial biotechnology, and environmental science, providing examples such as gene therapy, genetically modified crops, enzyme production, and bioremediation, with a focus on its role in vaccine and therapeutic protein development. Recent trends, including advancements in CRISPR and synthetic biology, are also discussed, along with potential future directions and ethical considerations. Essential laboratory practices, or Good Laboratory Practices (GLP), are emphasized, including safety protocols, documentation, quality control, and contamination prevention. Participants learn to handle basic laboratory instruments like micropipettes, centrifuges, and electrophoresis apparatus, along with fundamental calculations for solution preparation, such as percentages, molarity, and normality. Precise pipetting techniques, both forward and reverse, are taught for accurate liquid handling. The module covers competent cell preparation, explaining the role of competent cells in transformation, along with methods for making chemically and cells. An introduction to ligation techniques is provided, with practical training in T4 DNA ligation of λ DNA digested with HindIII. An overview of agarose gel electrophoresis follows, covering gel preparation, sample loading, and DNA visualization. This includes preparing tank buffers like TBE or TAE, making 6X loading dye, and safely handling ethidium bromide for DNA staining. To assess ligation efficiency, participants analyze gels, interpret band patterns, and examine expected results. The transformation of ligated DNA into competent cells is demonstrated, along with an overview of transformation techniques and vector types. The restriction-digestion technique is also introduced, with practical exercises involving enzymes like EcoR1 and HindIII to cut plasmid DNA, followed by gel electrophoresis analysis of digested fragments. Finally, the module delves into the basics of DNA fingerprinting using restriction fragment length polymorphism (RFLP) and blue-white screening for identifying recombinant clones, providing participants with a comprehensive hands-on experience in RDT essential for molecular biology and biotechnology applications.

Proteomics ( Module 12 )

The curriculum begins with an introduction to proteomics and its applications, followed by an emphasis on Good Laboratory Practices (GLP) to ensure safety and reliability in experiments. Participants will learn the basics of instrument handling essential for laboratory work and the fundamental calculations required for experiments, including percentage, molarity, and normality. The course includes practical training in reagent preparation and micropipette handling techniques, specifically focusing on both forward and reverse pipetting methods. Various basic tests for protein analysis will be covered, alongside screening techniques for identifying protein-rich sources for estimation. Students will gain an understanding of the principle and standard operating procedures (SOPs) associated with using a spectrophotometer for protein estimation, including estimation through UV-visible spectroscopy. Training will involve preparing standard curves and interpreting data effectively. The curriculum also addresses the extraction of proteins from various sources and different methods for protein precipitation. Participants will learn the preparation of reagents required for SDS-PAGE, which includes creating stacking gels, running gels, sample loading dye, tank buffers, staining solutions, and destaining solutions. The course will guide students through the process of protein separation using the vertical gel electrophoresis technique (SDS-PAGE), along with the concepts of staining and destaining in this method. Practical exercises will include staining and destaining SDS-PAGE gels, allowing for direct observation of results. Finally, an overview of protein analysis using the Western blotting technique will be presented, highlighting the differences between SDS-PAGE, Native PAGE, and 2D PAGE (T), providing students with a comprehensive understanding of protein analysis methodologies in a laboratory setting

Diabetes Single Nucleotide Polymorphism (SNP) Mutation Analysis ( Module 13 )

Introduction and scope of Molecular Biology, Overview of genomic DNA extraction, Bio-safety rules for working in a clinical lab, Demonstration of module-oriented instruments, Basic concept of molecular biology mathematics, Overview of Applied Molecular Biology, Basic handling and disposal of potential infectious/ clinical waste, Genomic DNA extraction from Blood, DNA sample quantification and qualitative analysis, Difference between Single Nucleotide Polymorphism and Mutations (Basic concept), Genetic risk factors for Diabetes and Molecular Diagnostics, SNP Polymerase Chain Reaction (SNP-PCR), SNP-PCR Amplicon gel electrophoresis, Data compilation, recording and interpretation, SNP analysis: Programs for prediction of Single Nucleotide Polymorphism effects. (Available at- NOIDA, Ahmedabad, Mumbai, Hyderabad, Jaipur and Dehradun)

Enzyme and Microbial Technology ( Module 14 )

Introduction and application of enzymology, Enzyme market size & major companies in India, Good Laboratory Practices (GLP), Basic instrument handling, Micropipette handling (forward or reverse pipetting techniques). Concept of basic calculation of percentage, molarity, and normality, Classification of media and solution preparation, Principle-standard operation procedure and application of instruments (pH meter, autoclave, colony counter, and laminar airflow), Screening of microbes producing extracellular hydrolytic enzymes (Amylase/Protease/Lipase/Tannase etc.) from different source (soil/water/industrial effluent etc). Subculturing of enzyme producing bacteria on culture media, Preparation of seed culture for fermentation media, Overview of fermentation and amylase enzyme, Preparation of fermentation media for amylase enzyme, Production of enzyme using submerged fermentation method (for amylase enzyme). Extraction of crude amylase enzyme, Determination of amylase enzyme activity using biochemical test, Enzyme/cell immobilization technique through sodium alginate and calcium chloride method. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Food Microbiology ( Module 15 )

Introduction to food microbiology and Microbial Limit Test (MLT), Good laboratory practices (GLP), Basic instrument handling and micropipette handling (forward and reverse pipetting techniques), Application and calibration/validation (Autoclave, pH Meter, Laminar Air Flow, Colony Counter, Incubator, and Microscope), Review of microbiological logbook entries, Concept of basic calculation (percentage, molarity, and normality), Types of media and solution preparation, Overview and types of sterilization techniques, Classification of bacteria based on Gram staining, Preparation of solid media for Diarrhea-causing bacteria, Screening of Diarrhea-causing bacteria on selective media from spoiled food samples, Introduction about streaking and subculturing, Streaking technique used for isolation of bacteria. Characterization of Diarrhea causing bacteria by staining technique, Basics of biochemical test, Identification of isolates using different biochemical test (Indole test/ Citrate test/ Urease test etc.), Antimicrobial susceptibility test against Diarrhea causing bacteria, Methylene Blue Reductase Test (MBRT) in milk. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Biochemistry & Immunology ( Module 16 )

Introduction to Biochemistry, Difference between antigen-antibody, Types of antibodies and basic instrument handling, Calculation (Percentage, Molarity and Normality), Micropipette handling (Reverse and Forward pipetting), Standard operating procedure (SOP) and application of different instruments (pH meter and spectrophotometer), Measurement of рН of biological liquids with universal indicator paper, Qualitative determination of Vitamin (A/C/D), Biuretic reaction for detection of protein, Qualitative test for the presence of carbohydrates (Molisch/ Tollen’s/ Fehling test), Quantitative determination of vitamin Р in the different samples via UV-VIS Spectrophotometer, Determination of alpha-Amylase activity (Serum/Saliva) on Starch, Components of blood. Bleeding time calculation, Clotting time estimation. Separation of Serum from human blood, Basic concept of antigen and antibody, Overview of enzyme-linked immunosorbent assay (ELISA), Direct Immuno enzymatic determination of thyrotropin/Hepatitis B in human serum through (ELISA) reader, Scientific approaches of ELISA and RIA technique. (Available at- NOIDA, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur and Dehradun)

Applied Industrial Microbiology ( Module 17 )

Industrial microbiology application & major products, Major companies in India, Scope & job prospects, Good laboratory practices (GLP), Demonstration of module-oriented instrument, Different types of sterilization techniques in microbiology, Basic principle-Standard Operating Procedure (SOP) and Application of the instruments (Autoclave, pH meter, Laminar air flow, incubator, microscope and colony counter), Classification of bacteria based on Gram staining, Types of media and solution preparation, Basic calculation (Percentage, Molarity and Normality), Overview of serial dilution, Serial dilution of soil sample, Basic calculation of Colony.

Bioinformatics (Module 18)

Introduction of bioinformatics, Application of bioinformatics, Scope of bioinformatics, Uses &Importance of bioinformatics, Sequence database similarity searching tools: FASTA, BLAST (BLASTP, BLASTN, BLASTX, TBLASTX, TBLASTN), Multiple sequence alignment tool: ClustalW, Introduction of Biological databases: Primary databases & Secondary databases, Sequence databases (GenBank, EMBL, DDBJ), Protein structure, classification and family databases (PDB, CATH, SCOP, Pfam, PIR,PROSITRE, Swiss-Prot), Detailed Understanding of Phylogenetic Analysis. 

Microbiology (Module 19)

Basic of Microbiology, Good laboratory practices (GLP), concept of basic calculation, solution preparation, media preparation, Overview and types of Sterilization techniques, Basic principles, standard operating procedure (SOP) and application of instruments (Autoclave, pH meter, Laminar air flow, incubator and microscope) Classification of bacteria on the basis of Gram staining, types of media, Difference between gram positive and gram negative bacteria, Solid and liquid media preparation, Serial Dilution techniques, Isolation of bacteria from soil sample, Isolation of pure bacterial colonies, Gram staining technique for bacteria, Motility check with hanging drop method, Lactophenol Cotton Blue (LPCB) staining for fungus, Methylene Blue Reductase Test in Milk.

Molecular Techniques (Module 20 )

Basic of Molecular Biology, Good laboratory practices (GLP), Overview of genomic DNA, Basic instrument handling, Basic concept of Percentage, Basic concept of Molarity and Normality, Micropipette handling (forward and reverse pipetting techniques), Principal, SOP and application of spectrophotometer, Composition of buffers, Extraction of genomic DNA from onion/banana, Extraction of genomic DNA from Bacteria, Extraction of genomic DNA from Plant, types of electrophoresis, Qualitative Analysis of DNA-Agarose Gel Electrophoresis, Quantitative Estimation of the DNA through UV-VIS spectrophotometer, RNA extraction (T) (N,M, L,Mu,Ah,,B,H,C,D)

Natural Product Research (Module 21)

Basics concepts of Natural Product Research , Safety of herbal medicine, Efficacy of herbal medicine, General and safety rules for working in Lab, Basic instrument handling, Concept of basic calculation, solution preparation, types of extraction, Preparation of standardized extracts through maceration, Introduction about phytochemicals, Qualitative Phytochemical analysis (saponin, tannin, flavonoids and alkaloids) of herbal extracts, types of media, Media preparation and Antimicrobial susceptibility tests (AST) of plant extracts against bacteria, Basic concept of Minimum Inhibitory Concentration test, Basics of chromatography, Thin layer chromatography (TLC) of crude extracts, Antioxidant Potential analysis of Bioactive extracts.

Food Technology (Module 22)

Basic of food technology, Good laboratory practices (GLP), Demonstration of instruments, concept of basic calculation, Micropipette handling (forward and reverse pipetting techniques), To extracts caffeine using polar-nonpolar solvent extraction technique and its confirmatory test, quality analysis of different Fruit Juices (pH, total moisture, solid content), overview of adulteration and this disadvantages according to FSSAI guidelines, Adulterant analysis in different brands of milk (Starch test, water test, formalin test, soap test and sulphate), Spectrophotometric Quantification of carbohydrates in different food samples, Quality analysis of turmeric powder (metanil yellow, yellow lead salt and starch test), Basic concept of chromatography, Thin Layer Chromatography (TLC). 

Biochemistry (Module 23)

Basic of Biochemistry, Good laboratory practices (GLP), Basic instrument handling, Concept of basic calculation, Micropipette handling (forward and reverse pipetting techniques), To perform the isoelectric precipitation of casein present in milk, Introduction and types of titration, To Estimate the Saponification value in fats/oils, Classification of carbohydrates, Qualitative analysis of carbohydrates, Analysis of lactose by mucic test and microscopic view of mucic acid crystals, Biuretic reaction for detection of peptide bond, Principal of DNS reagent, Principle, SOP and application of spectrophotometer, Quantitative estimation of Carbohydrate by DNS Method, standard graph preparation and data interpretation.